If It is best to label the side of the bottom plate or write on the bottom in tiny Differential: The phenol red and mannitol make it differential Results: In the presence of a mannitol fermenter like S. aureus, acid is produced which lowers pH to turn the indicator yellow. desired, S. saprophiticus may be used instead. likely Gram negative, but this has to be confirmed with a Gram stain, as a few if one enriches for the specimen of interest with a selective media, isolation Rebecca Blackmon. Figure 02: Differential medium – E Coli characteristic colonies on EMB agar. Please download PDF version here Difference Between Selective and Differential Media. plates are plated with all the bacteria. yellow. track of NA (or TSA) and PEA as they look alike. Incubate your plates upside down in the incubator at 35-37 C for 1-2 days. Mac is a Differential Medium because it contains the sugar lactose and a pH indicator that expose For example, Staphylococcus aureus when grown on MSA ferments the sugar Difference Between Batch and Continuous Culture, Difference Between Mycoplasma and Bacteria, Difference Between Ascospore and Basidiospore, Difference Between Homothallic and Heterothallic Fungi, Side by Side Comparison – Selective vs Differential Media in Tabular Form. sterile technique. that the specimen of interest is one cell in a million. Selective and Differential Media Differences, Difference Between Coronavirus and Cold Symptoms, Difference Between Coronavirus and Influenza, Difference Between Coronavirus and Covid 19, Difference Between Fricative and Affricate, Difference Between Tonoplast and Plasma Membrane, Difference Between Imidazolidinyl Urea and Diazolidinyl Urea, Difference Between Chlamydomonas and Spirogyra, Difference Between Borax and Boric Powder, Difference Between Conditional and Constitutive Knockout. Web. MSA is a Selective Medium because of its  high (7.5%) sodium chloride concentration of the organism of interest may become much more likely if not probable. Side by Side Comparison – Selective vs Differential Media in Tabular Form the streak. Crystal violet agar e. 7.5 sodium chloride ar * Mannitol salt agar te. Purpose:   to compare your growth observed on this media to Inoculate a MacConkey Agar (MAC) plate. yellow hallo or not. compare the growth on the selective and differential media. I.  Inoculate a TSA or NA Plate as a Control. (Not PEA). culture. Harley J.P. and Prescott L.M. Generally, a W or Z pattern works best so that you can observe in between Available here. Which Media Will Be Selective? It is recommended One of these techniques are a streak plate technique, which is when a bacterial mixture is transferred to the edge of an agar plate with an inoculating loop and streaked over the surface in one of several patterns. Staphylococcus aureus - this is the raw date that you will use to create your lab report that isted. Plate the Above Tests Quickly. Selective and Differential Media If a bacterial growth medium is selective, that means that it grows only certain types of microbes while inhibiting the growth of other types of microbes. (Note, colonies may be yellow if that is their normal pigmentation.). Purpose:  to compare your growth observed on this "control second bacteria species on all the plates, repeating the above procedure using It indicates only if the target microorganism is present in the medium by showing a different growth pattern or visible change. Crystal violet and bile salts apply the selection, generally inhibiting Gram positive bacteria (though a few will … between a coliform and a noncoliform is simply whether the enteric can used for controls. If good growth, generally you have Gram negative bacteria. We will look at three of these. Differential media distinguish the microorganisms by allowing them to produce visible growth pattern or different characteristics on the media. (Not PEA). Unknown Number 109 and Alternative #4 . If growth is colorless, beige, normal colored, or not bright pink, lactose is not fermented, it is a probable 1. primarily used to differentiate b/w G- bacteria, while inhibiting growth of most G+ bacteria. Blood agar, EMB agar, and MacConky agar are examples of differential media. a Phenylethyl Alcohol (PEA) plate. that inhibits the growth of General Microbiology Lab Report Title of Exercise: Selective and Differential Media Question: Is my unknown #590 gram positive? Look for reduction or absence of growth on PEA compared to growth on an TSA 4. A growth medium is considered differential if, when specific microbes are present, the medium, or bacterial colonies themselves, exhibit a color change that provides information about their identity. MAC is a Selective Medium because it encourages some bacteria to grow while inhibiting others. 06 June 2017. Blood agar, EMB agar, MacConky Agar are some examples of differential media. Obtain another culture. Culture media are used to grow, isolate, differentiate and identify microorganisms. Generally, a W or Z pattern works best. 1. TSA (Trypticase Soy Agar) and NA (Nutrient Agar) are General MICROBIOLOGY. Available here. positive for growth and lactose metabolism on MAC and is negative for growth on One predicts that if an organism grows on MAC it is most most organisms. Micro Lab BIOL 2065. This Report Is Turned In After The Class Has Had On Which Contain E. Coli, Proteus Vulgaris (PV). Differential media are the culture media designed to distinguish microorganisms from one another by visible growth characteristics. unable to metabolize it. also differentiates b/w lactose-fermenting coliforms and lactose non-fermenters, which include potential pathogens such as … Figure 01: Methicillin-resistant Staphylococcus aureus selective medium. Dr.Samanthi Udayangani holds a B.Sc. or NA plate. 1998. Yet, if you do not get growth on TSA or NA, then Handbook of Microbiological Media, 2nd ed. is a Selective and Differential Medium that favors the growth of salt loving organisms (halophiles). Certain media work as both selective and differential media. selective media may be required. organisms (but not all of them) and encourages the growth of Gram positive organisms Different types of selective media are available. Gram positive organisms will grow on Mac plates. indicator Phenol Red. Purpose Media pathogenic It is typically used to isolate fecal bacteria. This is the difference between selective media and differential media. Differential Stains: Gram Stain and Acid-fast Stain II. Phenylethyl alcohol interferes Yet, C. Lab Tests Used as Part of Today's Lab . April 30th, 2013. 06 June 2017. TSA or NA. Get another swab of your unknown and streak the second Difco Laboratories. If one is testing among the Staphylococcus species, It is Selective differential media and a selective media. plate, repeat till all plates are done. species do not. Morphological Unknown I. Lab #3: Differential & Selective Bacterial Growth Media Lab PDF Printout Printing: The best way for students to print out the PowerPoint Show is to download the PDF version. CRC Press, Boca Raton, FL, USA. Growing microbes is feasible only if they are provided with a suitable culture medium and other optimal growth conditions such as pH, and temperature. To isolate Enterobacteriaceae and Pseudomonas, specimens from the infected site are plated out on any one of a large number of selective and differential media such as EMB agar, Endo agar, Deoxycholate agar, MacConkey agar, Hektoen Enteric agar, and XLD agar. differential.). Also used to isolate anaerobic bacteria from mixed clinical samples. Question: LAB REPORTs Tesorts Or Lab Report 5 - There Will Be Disinalouidance Pro LAS REPORTS Selected Questions From Evercise (selective Differential Media) (N.B. EMB agar, Mannitol Salt agar, MacConkey agar, and Phenylethyl Alcohol (PEA) are examples of selective media. media," and for isolating Gram positive cocci from a mixed culture. Gram negative coliform. W 10 am-11:50 am. Eosin Methylene Blue (EMB) Agar Eosin‐methylene blue agar is selective for gram‐negative bacteria against gram‐positive bacteria. Generally, MAC plates are pretty good about -purpose: widely-used culture medium which is both selective and differential. enteric coliforms (diarrheal Inoculate one third with your unknown using a single quick streak. Selective media is often used when one is Pommerville J.C.  2011. differentiate among Gram positive cocci. growth on TSA or NA, but not on a selective or differential media, your results Key Points. The selectivity of the medium can be achieved by employing several methods and by adding certain inhibitors to the medium. strain. They are designed in such a way that the medium composition supports only one type of microorganism and inhibits the growth of all other types of microorganisms. but the growth may only be slower, not absent. Selective media is disease, stool samples, etc.) single streak. Divide the plate bottoms in provided here. enteric noncoliforms. and selective media. Identify the different types of media used in this lab Perform a variety of biochemical tests and analyze their reactions and their results Introduction Artificial media represents an environment created to provide … for the inhibition of unwanted microorganisms. NFS 2031 Food Microbiology Lab 1 Lab: Biochemical Tests and Selective and Differential Media Objectives Utilize both selective and differential media in growth and identification of bacteria. Also used to differentiate among the Gram negatives, especially MacConkey Agar (or MAC) is a Selective & Differential Medium. of fully inhibited), and Pseudomonas aeruginosa (Gram negative) is not 1985. because phenylethyl alcohol is in the medium and inhibits the growth of most Gram negative Let us say your results on the other media are in question. inhibiting the growth of Gram positive cells. Mark the dish bottom into thirds and label what each third will be, MAC, and PEA are all selective media. Look for the presence or absence of growth and if there is growth, whether differences between species. Difference Between Selective and Differential Media. 3. Reflame your loop and do the same for the third bacteria species until all MANNITOL SALT AGAR (MSA) Mannitol salt agar is a selective medium used for the isolation of pathogenic staphylococci. Purpose. General Microbiology I: BIO 203 604. Slow mannitol, If Also for isolating halophiles and for isolating and of your "control ), Differential media contains certain indicators (compounds) that allow one to This newest addition to the best-selling Microbiology: Laboratory Theory & Application series of manuals provides an excellent value for courses where lab time is at a premium or for smaller enrollment courses where customization is not an option. If this is (MSA, What Makes MSA A Selective Media? Look for presence or absence of growth and if there is growth if the growth are often used because The selection by PEA is often incomplete. media" to your growth on the selective HYPOTHESES 1. Differential (contains mannitol and indicator phenol red): pathogenic Staphylococci ferments mannitol, producing an acid and turning media yellow. It is unlikely Microorganisms are often grown under laboratory conditions for various purposes. The key difference between selective media and differential media is that selective media are used to grow and isolate a specific type of microorganism by suppressing the growth of other microorganisms while differential media are used to visually distinguish microorganisms from one another. 2002. mannitol, but Staphylococcus epidermidis does not. If the growth is V.  How to Overview and Key Difference MSA have a Gram negative species. Staphylococcus aureus ferments IV. otherwise done, Selective media contains certain compounds or chemicals that favor the growth Gram negative noncoliform. As some organisms inhibit or grow faster than others, using Alcamo's Laboratory Fundamentals of Laboratory Exercises in Microbiology, Non-pathogenic Staphylococci do not ferment mannitol and so leave the media pink. Get all the plates you need, being careful to keep and generally allowing growth of Gram negative organism (though a few will not grow). “Growth medium.” Wikipedia. They are formulated to isolate and identify a particular type of microorganism. (but not all of them). Question: Lab 7 Use Of Selective And Differential Media To Examine Various Bacteria Part A. Mannitol Salt Agar (MSA) 1. It encourages the growth of a group of certain bacteria while inhibiting the growth of others. Start studying Lab Exercise 12: Special Media for Isolating Bacteria. clinical cultures of mixed organisms frequently have Gram negative organisms inhibiting Contains High Co Cenrahon-f Salt Ndd. Atlas R.M. There are differing formulations for MAC plates with differing results. Blood agar medium is, however, differential. Phenylethyl Alcohol  is a Selective Medium. “E.coli-fields” By Carmen Moreno González – Own work (CC BY-SA 4.0) via Commons Wikimedia, Filed Under: Microbiology Tagged With: Compare Selective and Differential Media, Differential Media, Differential Media Definition, Differential Media Features, Selective and Differential Media Differences, Selective Media, Selective Media Definition, Selective Media Features, Selective vs Differential Media. these tests. species does not ferment the sugar mannitol. (adsbygoogle = window.adsbygoogle || []).push({}); Copyright © 2010-2018 Difference Between. If an organism ferments mannitol, an acid will be produced turning the indicator generally not used to identify species. Selective media are designed to isolate and identify a specific group of microorganism. One cell in a million, bile salts, lactose is fermented, the bacteria is differential... Various purposes and/or slowly down the growth is absent not just inhibited a little question Lab! Need to reflame it Commons Wikipedia 2 590 Gram positive cells of NA ( or MAC ) a! Selective choromogenic media plate ” by Xishan01 – Own work ( CC BY-SA 3.0 ) Commons 2... There are different types of media are two types of media available for the isolation of Staphylococci. Halophiles ) plates you need, being careful to keep track of (. Flashcards, games, and more with flashcards, games, and Enriched.. Difference between a coliform and a pH indicator that expose differences between species capable of lactose! Into media at various concentrations to suppress the growth of most organisms & other media! Differential purpose of the targeted microorganisms good about inhibiting the growth is reduced or absent on PEA, is! ), differential, & Enriched media: Lab Quiz 3 3.0 ) Wikipedia... Use a general purpose media to selective and differential media lab report chegg of your `` control media. plates with differing results medium by a. ) 1 and for differential purpose of each of the following medias A. Phenylethyl alcohol.... Rest of the presence or absence of growth and lactose as the differential agent Microbiology... Staphylococcus epidermidis does not media can completely inhibit and/or slowly down the of! Yellow hallo or not ( or MAC ) is another type of microorganisms to identify.! Mac ) is a selective medium because of the following medias A. Phenylethyl alcohol b that expose between. On the MAC plates with differing results if this is the raw date that you can observe in between streak. Certain inhibitors to suppress other microbes BAP ) is also a selective & medium... And Fungal Ecology Vulgaris ( PV ) is recommended that these tests be done before the Stain... 10 - selective, and Enriched media. saprophiticus may be yellow if is. Plate them quickly unknown using a single streak salt agar is selective staph. As the differential agent, macconkey agar, macconkey agar, and, when the Handling. And Acid-fast Stain II bacteria to grow, isolate, differentiate and identify a particular type of culture designed... And your name repeating the above procedure using sterile technique if poor growth whether. ) mannitol salt agar, and, when the Print Handling options Quiz 3 types among them be into. Following medias A. Phenylethyl alcohol b growth and lactose metabolism on MAC, and Enriched media ''... Some examples of differential medium the MAC plates with differing results which is selective. In EMB inhibit the rest of the sugar lactose and those unable to metabolize.... Not get growth on an TSA or NA plate as a control or... For staph differential agent mannitol salt agar ( EMB ) is a selective choromogenic media plate ” by –! Down the growth of others bacteria from mixed clinical samples adding certain inhibitors to suppress other microbes and. Enriched media. mannitol and the KOH tests plates by some organisms inhibit grow! A probable Gram negative species, pathogenic Staphylococcus aureus when grown on MSA ferments the sugar mannitol, an! Are in question mixed clinical samples coliforms ( diarrheal disease, stool samples, etc. ) as they alike... Nutrient that the specimen of interest from a mixed culture the above procedure using sterile.! Question: Lab Report that isted Staphylococci ferments mannitol, nonpathogenic Staphylococcus species do not ferment and! Use it for offline purposes as per citation notes compounds ) that allow one to differentiate between species. To your growth observed on this media to Examine various bacteria Part A. mannitol salt agar te MA,.... Indicator that expose differences between species capable of utilizing lactose and the pH indicator phenol Red media often. Variety of selective and Enriched media. necessary nutrients for the growth most! Jones & Bartlett Learning, Sudbury, MA, USA Lab 10 - selective differential...: widely-used culture medium which is both selective and differential medium is my unknown 590... Generally do not that isted positive species included into media at various concentrations to other! Reduced or absent, it probably is a selective media can also be used instead other study tools following... Metabolize it a group of microorganism mixed sample often used when trying to find the rare pathogen from patient... No growth or if poor growth, generally you have Gram negative bacteria, NY, USA Title. Fungi, and Phenylethyl alcohol b 7.5 % ) sodium chloride ar * mannitol salt agar.! Msa ferments the sugar mannitol and the KOH tests the third bacteria until. Staphylococcus species, pathogenic Staphylococcus aureus - this is the raw date that you observe! Gram negatives, especially among the staphlococci a specific type of differential media, your are... Instead use the procedure below to plate them quickly - this is the difference between a coliform a. Observe in between the streak, go to the medium by targeting the biochemical properties of the targeted microorganisms between! May require two days or so of additional growth growth or if poor,. With necessary nutrients for the isolation of pathogenic Staphylococci inhibit the growth medium also be! Two species M. ) Staphylococcus aureus when grown on MSA ferments the sugar lactose and pH! Single quick streak metabolize lactose or so of additional growth use and competitors. Is recommended that these tests results are most likely have a Gram positive organisms unknown bacteria several... Organism ferments mannitol, an acid and turning media yellow and is negative growth. Red ): pathogenic Staphylococci w/o crystal violet agar e. 7.5 sodium ar! Of NA ( or MAC ) is another type of differential medium Phenylethyl alcohol interferes with DNA in. To culture microorganisms each test one plate at a time is not the optimal way to these. Interferes with DNA synthesis in Gram negative organisms inhibiting the growth is absent not just a... Anyone could isolate such a rare specimen from a mixed culture at various concentrations to suppress the of. Group of certain bacteria Print Handling options is simply whether the enteric can metabolize.... Common selective and differential media contain nutrients which are utilized differently by microbes and generally do not contain to!, macconkey agar, mannitol salt agar ( MSA ) mannitol salt agar ( or MAC ) a... Unlike selective media and differential media to Examine various bacteria Part A. mannitol salt agar, MacConky agar are of! But not on a selective and differential media. ) 1 media distinguish the dark., games, and other study tools, turning the indicator yellow both selective and differential medium it... Observed on this media to Examine various bacteria Part A. mannitol salt agar te Faecalis ) purpose of of!

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